Table 1.
Yeast strains used in this study
| Strain | Relevant genotypea | Reference/Source |
|---|---|---|
| SM1058 | MATatrp1 leu2 ura3 his4 can1 | Michaelis and Herskowitz, 1988 |
| SM1068 | MAT α lys1 | Michaelis and Herskowitz, 1988 |
| SM1646 | MATatrp1 leu2 ura3 his4 can1 ste6-Δ2::URA3 | Berkower and Michaelis, 1991 |
| SM1934 | MATaade2 leu2,3-112 his3 trp1 ura3 pep4::TRP1 | R. Schekman |
| SM2187 | MATaend4tsura3 his4 leu2 bar1-1 | Raths et al., 1993 |
| SM2544 | MATatrp1 leu2 ura3 his4 can1 ste6-Δ4 | Loayza et al., 1998 |
| SM2721 | MATatrp1 leu2 ura3 his4 can1 ste6-Δ5 | Berkower et al., 1996 |
| SM3205 | SM2721 [2μ LEU2 ste6-G414R::HAc] | Transformant of SM2721 with pSM1129 |
| SM3206 | SM2721 [2μ LEU2 ste6-G1092V::HAc] | Transformant of SM2721 with pSM1130 |
| SM3207 | SM2721 [2μ LEU2 ste6-L1239X::HAe] | Transformant of SM2721 with pSM1131 |
| SM3208 | SM2721 [2μ LEU2 ste6-G132R::HAc] | Transformant of SM2721 with pSM1132 |
| SM3209 | SM2721 [2μ LEU2 ste6-T1101R::HAc] | Transformant of SM2721 with pSM1133 |
| SM3210 | SM2721 [2μ LEU2 ste6-G38D::HAc] | Transformant of SM2721 with pSM1134 |
| SM3220 | SM2721 [2μ LEU2 STE6::HAc] | Transformant of SM2721 with pSM500 |
| SM3245 | SM1058 [2μ URA3 CFTR::HA] | Transformant of SM1058 with pSM1152 |
| SM3302 | SM2721 [2μ URA3 MDR1::HA] | Transformant of SM2721 with pSM812 |
| SM3317 | SM2544 [2μ URA3 ste6-Q1249X::HAe] | Transformant of SM2544 with pSM1082 |
| SM3897 | SM2721 [2μ URA3 STE6::GFPc] | Transformant of SM2721 with pSM1493 |
| SM3898 | SM2721 [2μ URA3 ste6-G38D::GFPc] | Transformant of SM2721 with pSM1508 |
| SM3863 | SM2187 [2μ URA3 STE6::GFPc] | Transformant of SM2187 with pSM1493 |
| SM3954 | SM2187 [2μ URA3 ste6-G38D::GFPc] | Transformant of SM2187 with pSM1508 |
| SM4031 | SM1934 [2μ URA3 STE6::GFPc] | Transformant of SM1934 with SM1493 |
| SM3966 | SM2721 [2μ LEU2 STE6::HAe] [2μ URA3 STE14] | Transformant of SM2721 with pSM694 and pSM1317 |
| SM3967 | SM2721 [2μ LEU2 ste6-L1239X::HAe] [2μ URA3 STE14] | Transformant of SM2721 with pSM1131 and pSM1317 |
| SM4031 | SM1934 [2μ URA3 STE6::GFPc] | Transformant of SM1934 with pSM1493 |
| SM4207 | SM2721 [CEN URA3 SEC63::GFP] [2μ LEU2 STE6::HAe] | Transformant of SM2721 with pSM1462 and pSM694 |
| SM4208 | SM2721 [CEN URA3 SEC63::GFP] [2μ LEU2 ste6-L1239X::HAe] | Transformant of SM2721 with pSM1462 and pSM1131 |
| SM4209 | SM2721 [CEN URA3 SEC63::GFP] [2μ LEU2 ste6-G38D::HAc] | Transformant of SM2721 with pSM1462 and pSM1134 |
| SM4213 | SM2721 [2μ URA3 ste6-G38D::GFPc] [2μ LEU2 STE14] | Transformant of SM2721 with pSM1508 and pSM1356 |
| SM4255 | MATaura3-52 leu2-3,112 ste6-Δ5 | This study |
| SM4256 | SM2721 [2μ LEU2 STE6::HAe] [2μ URA3 ste6-G38D::GFPc] | Transformant of SM2721 with pSM694 and pSM1508 |
| SM4460 | MATahis3 leu2 met15 ura3 | Invitrogen, Carlsbad, CA |
| SM4817 | MATahis3 leu2 met15 ura3 pep4::kanMX | Invitrogen, Carlsbad, CA |
| SM4821 | MATahis3 leu2 met15 ura3 ubc7::kanMX | Invitrogen, Carlsbad, CA |
| SM4922 | SM4460 [2μ LEU2 STE6::HAe] | Transformant of SM4460 with pSM694 |
| SM4923 | SM4460 [2μ LEU2 ste6-L1239X::HAe] | Transformant of SM4460 with pSM1131 |
| SM4924 | SM4460 [2μ LEU2 ste6-G38D::HAe] | Transformant of SM4460 with pSM1134 |
| SM4925 | SM4817 [2μ LEU2 STE6::HAe] | Transformant of SM4817 with pSM694 |
| SM4926 | SM4817 [2μ LEU2 ste6-L1239X::HAe] | Transformant of SM4817 with pSM1131 |
| SM4927 | SM4817 [2μ LEU2 ste6-G38D::HAe] | Transformant of SM4817 with pSM1134 |
| SM4928 | SM4821 [2μ LEU2 STE6::HAe] | Transformant of SM4821 with pSM694 |
| SM4929 | SM4821 [2μ LEU2 ste6-L1239X::HAe] | Transformant of SM4821 with pSM1131 |
| SM4930 | SM4821 [2μ LEU2 ste6-G38D::HAe] | Transformant of SM4821 with pSM1134 |
| SM4933 | SM4255 [CEN URA3 Pgal] [CEN LEU2 4 X UPRE-lacZ] | Transformant of SM4255 with pSM640 and pSM1408 |
| SM4934 | SM4255 [CEN URA3 Pgal STE6::HAe::GFPc] [CEN LEU2 4 X UPRE-lacZ] | Transformant of SM4255 with pSM1503 and pSM1408 |
| SM4935 | SM4255 [CEN URA3 Pgal ste6-G38D::GFPc] [CEN LEU2 4 X UPRE-lacZ] | Transformant of SM4255 with pSM1512 and pSM1408 |
a
HAc or GFPc (c for C-term) and HAe (e for ecto) refer to the presence of the epitope at the C terminus and in the first extracellular loop of Ste6p, respectively.