Table 2.
Plasmids used in this study
| Plasmid | Relevant genotypea | Reference/Source |
|---|---|---|
| pSM500 | 2μ LEU2 STE6::HAc | Paddon et al., 1996 |
| pSM640 | CEN URA3 Pgal | P. Hieter |
| pSM694 | 2μ LEU2 STE6::HAe | This laboratory |
| pSM812 | 2μ URA3 MDR1::HA | This study |
| pSM1082 | 2μ URA3 ste6-Q1249X::HAe | Loayza et al., 1998 |
| pSM1129 | 2μ LEU2 ste6-G414R::HAc | This study |
| pSM1130 | 2μ LEU2 ste6-G1092V::HAc | This study |
| pSM1131 | 2μ LEU2 ste6-L1239X::HAe | This study |
| pSM1132 | 2μ LEU2 ste6-G132R::HAc | This study |
| pSM1133 | 2μ LEU2 ste6-T1101R::HAc | This study |
| pSM1134 | 2μ LEU2 ste6-G38D::HAc | This study |
| pSM1135 | 2μ LEU2 ste6-G397D::HAc | This study |
| pSM1152 | 2μ URA3 CFTR::HA | Zhang et al., 2001a |
| pSM1317 | 2μ URA3 STE14 | Romano et al., 1998 |
| pSM1356 | 2μ LEU2 STE14 | This laboratory |
| pSM1408 | CEN LEU2 4 X UPRE-lacZ | Sidrauski et al., 1996 |
| pSM1462 | CEN URA3 SEC63::GFP | Prinz et al., 2000 |
| pSM1493 | 2μ URA3 STE6::GFPc | This study |
| pSM1503 | CEN URA3 PgalSTE6::HAe::GFPc | This study |
| pSM1508 | 2μ URA3 ste6-G38D::GFPc | This study |
| pSM1512 | CEN URA3 Pgalste6-G38D::GFPc | This study |
a
HAc or GFPc (c for C-term) and HAe (e for ecto) refer to the presence of the epitope at the C terminus and in the first extracellular loop of Ste6p, respectively.