Figure 4.

Decrease in the association of Rrn3p with Pol I after rapamycin treatment as analyzed by coimmunoprecipitation. Cellular extracts were prepared from NOY1170 cells carrying HA-tagged RRN3 treated with rapamycin for 1 h and from untreated cells. Pol I was immunoprecipitated with anti-A190 antibodies. Increasing amounts of the immunoprecipitated proteins (4, 6, and 8 μl) were subjected to Western blot analysis by using antibodies against the Pol I A135 subunit and the HA tag. Quantitation indicated that the amount of Rrn3p relative to Pol I in the immunoprecipitates for the rapamycin treated cells was 38% of that for the untreated cells.