Table 2.

Transcription of rRNA genes in the ≈40 copy yeast strain (NOY886) after rapamycin treatment and in post-log phase as analyzed by the EM Miller spreading technique

	Polymerase density			Inactive genes (inactive/active)
Samples	Ave.	Mode	N
Log phase (A600 0.42)	100 ± 29	114	174	<1%*
10-min rapamycin	60 ± 23	57	132	2/233
30-min rapamycin	36 ± 20	25	114	2/202
Post-log phase (A600 2.8)	30 ± 13	27	76	22/229

^*Although rare occurrences of inactive genes were observed, > 99% of rRNA genes were active as described previously (French et al., 2003).