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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: J Mol Cell Cardiol. 2012 Jan 3;52(3):596–607. doi: 10.1016/j.yjmcc.2011.12.012

Figure 2.

Figure 2

Species specific considerations in separating the SUR2A and SUR2B transcripts by RT-PCR. The C-termini of the mature mRNA of SUR2A and SUR2B of human and mouse origin are shown (ENSEMBL exon numbering). In order to separate between the two transcripts, different sets of primers need to be used in the case of mRNA of human origin, with the reverse primers designed in the last exon. For the mouse, the same set of primers can discriminate between the two transcripts if the two primers are designed across the alternatively spliced exon. The rat Abcc9 gene has a similar exonic organization as the mouse (not shown).