Figure 11. Brdu proliferation assay on activated CD8⁺ T cells.

BALB/c mice were immunized with 1 × 10⁵FFU BNSP-Gag-IFN(−) or BNSP-Gag-GM-CSF. One PBS mouse was added as a control. Three days post prime, the mice were injected i.p. with 2mg BrdU. For the following three days, the mice were given BrdU (0.8mg/ml) in their water. The spleens and ILNs were isolated from the mice 7 days post prime. Activated CD62L^lo CD8⁺ T cells were measured by flow cytometry (a). BrdU positive cells among the CD62L^lo CD8⁺ T cells are shown (b). Statistical analysis was performed using one-way ANOVA, * P <0.05, *** P<0.001.