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. Author manuscript; available in PMC: 2013 May 10.
Published in final edited form as: Virology. 2012 Feb 16;426(2):120–133. doi: 10.1016/j.virol.2012.01.025

Figure 7. Mice challenged i.p. with VV-Gag elicit a recall response proportional to the primary and memory immune profiles in the spleen.

Figure 7

Mice immunized with 1 × 105 FFU BNSP-Gag-IFN(−) and BNSP-Gag-GM-CSF were rested for 30 days. Each group was challenged with 1 × 106 FFU VV-Gag and the immune responses analyzed on day 3, 4 and 5 as shown (a). Gag-specific CD8+ T cells in the lymph nodes (b) and spleen (c) were analyzed and presented as a percentage. The functional expression of the CD8+ T cells was further monitored in an IFN-γ ELISpot assay following AMQMKLETI peptide stimulation (d). Statistical analysis was performed using one-way ANOVA, P <0.05, ** P<0.01. The data are representative of three repeat experiments.