Figure 6. AD brains had elevated protein phospho-tyrosine and active phospho-Src levels compared to age-matched controls.
AD and age-matched control (c) temporal lobe lysates (n=6) were resolved by 10% SDS-PAGE and Western blotted using (A) anti phospho-Tyr (4G10), anti-Aβ (6E10), anti-α-tubulin (loading control) antibodies, (C) anti-Lyn, anti-phospho-Lyn (Tyr396), anti-Src, and anti-phospho-Src antibodies. Optical density of (B) Total phospho-Tyr or Aβ multimers and (D) pLyn and pSrc from 6 AD and age-matched control brain blots were normalized to their respective α-tubulin blots or total protein levels (Lyn/Src) then averaged and graphed (+/−SD). (*p< 0.001)