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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Dev Biol. 2012 Jan 27;364(1):77–87. doi: 10.1016/j.ydbio.2012.01.017

Figure 2.

Figure 2

Quantitative evaluation of Delta/Notch perturbations. (A, B) RNA from Delta MASO or Notch MASO injected, and DAPT treated embryos was extracted and quantified using the Nanostring nCounter. The counts obtained for each gene in the codeset in perturbed embryos are plotted against those of control embryos. Perturbation with Delta MASO or Notch MASO produces almost identical results at 15 hpf, except minor differences that are not substantiated in repeat experiments. Only five genes are reproducibly affected indicating that Delta/Notch signaling has a small number of direct targets. (C, D) Application of DAPT, a Notch inhibitor, at 3 hpf produces results equivalent to Delta MASO treatment. At 24 hpf essentially all mesodermal genes included in the Nanostring codeset are affected by both perturbations. The dotted lines indicate a threshold of 2-fold change. Transcription levels were estimated from previous quantification data (Materna et al., 2010); genes present with 25 transcripts or less per embryo are marked with an open, grey circle.