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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Dev Biol. 2012 Jan 27;364(1):77–87. doi: 10.1016/j.ydbio.2012.01.017

Figure 4.

Figure 4

Effect of Notch MASO treatment on transcript levels. The results are essentially identical to Delta MASO treatment (see Fig. 3): (A–D) Mesodermal genes, and foxY in the small micromeres, are strongly affected by Notch MASO injection while endodermal genes are not affected. (C, D) In contrast to Delta MASO injection, which causes an upregulation of delta transcripts at 18 hpf and 24 hpf (see Fig. 3 C,D), Notch MASO has no effect on delta expression. (D) At 24 hpf the apical genes ac/sc and z133/fez exhibit increased abundance. This effect is stronger in Notch MASO injected embryos (and DN-Su(H) expressing embryos; see Fig. 5D) as compared to Delta MASO injected embryos, where these transcripts are slightly down-regulated (Fig. 3D). The exact spatial relationship between ac/sc, z133/fez, and delta expression is currently unknown just as their connection to other regulatory genes in the apical domain. z133/fez has recently been shown to antagonize Bmp signaling in the apical domain (Yaguchi et al., 2011). Data were acquired and analyzed as for Delta MASO. Thresholds and symbols are as in Fig. 3.