Figure 6. rFIZZ1 binds to leukocytes and structural cells isolated from naïve WT mouse lung.
Single cell suspensions from two naïve WT mouse lungs were incubated with rFIZZ1 followed by staining as shown (A). Lung cells were stained with CD45 and CD11c. CD45 positive cells (left) were gated and CD11c+ and CD11c− populations (right) were analyzed for FIZZ1 binding (B). CD45− cells were gated analyzed for FIZZ1 binding (C). CD45− cells from Col-1 GFP mice were gated on collagen-1 positive cells and analyzed for FIZZ1 binding (D). Lung cells analyzed from pooled samples from two mice and are results of two independent experiments.