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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: J Immunol. 2012 Feb 8;188(6):2493–2497. doi: 10.4049/jimmunol.1101502

Figure 3. Enhanced cytokine expression in Cradd-deficient mice, isolated spleen cells, and CD4+T cells.

Figure 3

Cytokines produced in mice following IV injection of ConA (A) or by spleen cells following stimulation with 1 μg/ml Con A (B) or with plate-immobilized anti-CD3 (2 μg/ml) and anti-CD28 (1 μg/ml) antibodies (C), or purified CD4+ T cells stimulated with anti-CD3/CD28 antibodies (D) were determined from wild-type (solid squares) and homozygous mutant mice (open circles). The levels of IL-6, MCP-1, IL-2, IFN-γ and TNF-α in plasma, and IL-2, IFN-γ, IL-17 and TNF-α in cell culture supernatants were measured by CBA assay. Mean values (± S.D.) for 9 independent experiments (A–C) or 3 experiments (D) are plotted. (*, <0.05; #, <0.005; and ^, <0.0005, repeated measures ANOVA with Bonferroni post-test).