Figure 7.
RV1B infection of 7-day old BALB/c pups induces persistent increases in the expression of mucus-related genes (A–C) and IL-13 (D). mRNA expression was measured by qPCR and normalized for GAPDH. N = 4 animals per time point, *different from sham-exposed mice of the same age, p<0.05, unpaired t test. E. IL-4, IL-5 and IL-13 protein levels from the lungs of RV-infected mice harvested three weeks after neonatal infection were significantly elevated compared to sham-exposed mice. Protein levels were measured by immune bioplex assay. (N = 4–5 animals per group, *different from sham-exposed mice, p<0.05, unpaired t test).