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The effect of acidic pericellular pH (A) or alkaline pHe (B) on buffering capability of N2a at 24 h. The data represent the optical density for phenol red indicator dye over a wavelength scan 500–600 nm in media blanks (left panel), presence of cells (middle panel) and a 550 nm comparison for media blanks versus cell supernatant across pHe. The data are presented by the mean ± SEM, n = 4 (right panel).
