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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: J Mol Cell Cardiol. 2011 Dec 1;52(3):638–649. doi: 10.1016/j.yjmcc.2011.11.011

Figure 8. Desumoylation of SUMO substrates in SENP2-Tg hearts.

Figure 8

Co-immunoprecipitation (IP) was performed on the whole tissue extracts from age-matched WT and SENP2-Tg mouse hearts using agarose-conjugated anti-SUMO-1 antibody for pulldown; subsequently, immunoblot (IB) was performed using anti-RanGAP1 (A) or anti-GATA4 antibodies (B) for detection. Arrows indicate SUMO-1-conjugated RanGAP1 (A) and SUMO-1-conjugated GATA4 (B). Note the substantial decrease in the level of SUMO-1 conjugated RanGAP1 and GATA4 in SENP2-Tg heart compared with those in WT heart.