Table 3.
Response to repeated hypoxia by control and Tfr2/Tmprss6 double mutant mice.
| strain | Normoxia Mean ± SE (n) |
3 × Hypoxia Mean ± SE (n) |
P value no hpx (Cont vs mut) |
P value 3x Hpx (Cont vs mut) |
P value control (no vs 3x Hpx) |
P value mutant (no vs 3x Hpx) |
|
|---|---|---|---|---|---|---|---|
|
Hemoglobin (g/dL) |
AKRB6 |
12.1 ± 0.7 (8) |
14.3 ± 0.6 (8) |
||||
| Tfr2Y245X/Y245X/Tmprss6msk/msk | 10.4 ± 0.1 (8) | 10.6 ± 0.5 (8) | 0.0133 | 0.002 | 0.0356 | 0.4913 | |
| MCV (fL) | AKRB6 | 45.6 ± 2.5 (8) | 49.3 ± 1.3 (8) | ||||
| Tfr2Y245X/Y245X/Tmprss6msk/msk | 28.8 ± 0.5 (8) | 37.9 ± 0.6 (8) | 0.0027 | 0.0009 | 0.1557 | 0.0009 | |
| Hematocrit (%) | AKRB6 | 39.6 ± 2.1 (8) | 47.4 ± 2.3 (8) | ||||
| Tfr2Y245X/Y245X/Tmprss6msk/msk | 39.0 ± 0.9 (8) | 41.1 ± 1.5 (8) | 0.3823 | 0.0585 | 0.0458 | 0.1949 | |
| MCH (pg) | AKRB6 | 13.9 ± 0.8 (8) | 14.9 ± 0.4 (8) | ||||
| Tfr2Y245X/Y245X/Tmprss6msk/msk | 7.7 ± 0.4 (8) | 9.7 ± 0.3 (8) | 0.0013 | 0.0009 | 0.0806 | 0.0073 | |
| RDW (%) | AKRB6 | 17.9 ± 0.5 (8) | 21.7 ± 1.3 (8) | ||||
| Tfr2Y245X/Y245X/Tmprss6msk/msk | 24.5 ± 0.4 (8) | 33.5 ± 0.7 (8) | 0.0009 | 0.0009 | 0.0051 | 0.0002 | |
| RBC (M/µl) | AKRB6 | 8.8 ± 0.3 (8) | 9.7 ± 0.6 (8) | ||||
| Tfr2Y245X/Y245X/Tmprss6msk/msk | 13.6 ± 0.5 (8) | 10.9 ± 0.4 (8) | 0.0002 | 0.1049 | 0.5737 | 0.003 | |
|
Liver Iron (mg/g) |
AKRB6 |
0.39 ± 0.1 (8) |
0.74 ± 0.1 (8) |
||||
| Tfr2Y245X/Y245X/Tmprss6msk/msk | 0.29 ± 0.0 (8) | 0.78 ± 0.1 (8) | 0.3823 | 0.7209 | 0.0281 | 0.0006 | |
|
Spleen Iron (mg/g) |
AKRB6 |
4.3 ± 0.8 (8) |
7.2 ± 1.0 (8) |
||||
| Tfr2Y245X/Y245X/Tmprss6msk/msk | 2.9 ± 0.3 (8) | 3.6 ± 0.5 (8) | 0.1275 | 0.0104 | 0.0379 | 0.1560 | |
Mice were exposed to 8% oxygen under normal pressure three times for 8 consecutive days separated by 2 weeks of normoxia. Means and standard errors are shown. Eight mice were used in each group. Statistical significance was determined using Mann Whitney test (Graphpad Prism 5.0).