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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Dec 17;1822(4):557–563. doi: 10.1016/j.bbadis.2011.12.005

Figure 1. Hypericin and Hyperforin do not inhibit adipogenesis.

Figure 1

3T3-L1 preadipocytes, induced to differentiate using the typical MDI cocktail, were treated at the time of induction with DMSO vehicle (V), methanol vehicle (M), 50 μg/ml flower (F) and root (R) extracts of SJW, or the indicated the doses of hypericin (HI) and hyperforin (HF). The cells were retreated following each media change every 48–72 hours. Control cells (C) received no treatment. Each panel is representative of 3–6 independently performed experiments. A: Oil Red O staining of neutral lipid content was performed 7 days post-MDI. All of the wells shown were stained at the same time with the same Oil Red O working solution. B: Western blot analysis of whole cell extracts from cells harvested four days post-MDI. Similar results were obtained at 4 and 7 days post-MDI, and panel B contains a representative subset of these blots.