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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Dec 17;1822(4):557–563. doi: 10.1016/j.bbadis.2011.12.005

Figure 3. SJW flower extract modulates insulin action via attenuation of IRS-1 tyrosine896 phosphorylation.

Figure 3

A: Mature 3T3-L1 adipocytes were serum deprived and pre-treated with DMSO vehicle (V) or 25 μg/ml SJW flower (SJW-F) extract overnight. Two independently performed experiments with the flower extract are shown, and this experiment was independently repeated a total of 2 times. B: Fully differentiated subcutaneous human adipocytes were serum deprived and pre-treated with 25 μg/ml SJW-F for the indicated lengths of time. Cells were then stimulated with insulin prior to preparation of whole cell extracts. Proteins from the whole cell extracts were resolved and immunoblotted. For each treatment and/or time point in B, data from two separate wells treated with insulin are shown. STAT5A levels are not altered by acute insulin stimulation, and its expression was examined as a loading control.