Table 1.
C. elegans immunity pathway components (adapted from Schulenburg and Boehnisch, 2008), H. sapiens orthologs, and L. oneistus clusters encoding for putative orthologs. Isogroup abundance indicates the number of sequences making up each cluster (when more than one cluster is present, the number of sequences making up each cluster were added and the total number is displayed); isogroup abundance may be directly correlated to transcript abundance. 454 transcriptomic data generated from symbiotic L. oneistus adults is available at: http://genepool.bio.ed.ac.uk/GP_Partigene/2008075_SilviaBulgheresi/; 14,747 putative genes (11,368 contigs and 3,379 singletons) corresponding to 5,888,206 bases were identified
| C. elegans | H. sapiens | L. oneistus ortholog-encoding clusters | Isogroup abundance |
|---|---|---|---|
| TGF-beta pathway | |||
| Neuron | |||
| DBL-1 | TGF--β ligand | LOP04675 | 3 |
| Epithelial cell | |||
| SMA-6 | Type I TGF-β receptor | LOP07766 | 1 |
| SMA-2 | Smad protein | LOP03197 | 1 |
| SMA-3 | Smad protein | LOP03388 | 2 |
| SMA-4 | Smad protein | LOP06423 | 1 |
| Insulin/IGF-1 (IIS) pathway | |||
| Neuron | |||
| GOA-1 | G protein α subunit | LOP05008 | 2 |
| DGK-1 | Diacylglycerol kinase -β | LOP03176, LOP07534 | 2 |
| Epithelial cell | |||
| DAF-2 | Insulin/IGF-1 receptor | LOP06243 | 1 |
| AGE-1 | Phosphatidylinositol 3-kinase | LOP04446, LOP08838, LOP07119 | 3 |
| AKT-1/AKT-2 | Rac Ser/Thr protein kinase | LOP05995, LOP09110, LOP06152 | 5 |
| SGK-1 | Serum/glucocorticoid regulated kinase 1 | LOP05995, LOP09110 | 3 |
| DAF-16 | FOXO family transcription factor | LOP10192, LOP07765 | 5 |
| P38 MAPK pathway | |||
| Epidermal cell | |||
| RACK-1 | G protein β subunit | LOP01842 | 9 |
| PLC-3 | Phospholipase C γ | LOP05069 | 2 |
| PKC-3 | Protein kinase C iota type | LOP11897 | 2 |
| Intestinal cell | |||
| EGL-30b | G protein G(q) α subunit | LOP06585, LOP05008 | 4 |
| DKF-2 | Ser/Thr protein kinase D | LOP07419, LOP10773 | 4 |
| NSY-1 | ASK1 MAPKKK | LOP04342a, LOP09080a | 5 |
| SEK-1 | MKK3, MKK6 MAPKK | LOP05076, LOP05850, LOP07733, LOP02761 | 7 |
| PMK-1 | p38 MAPK | LOP01689, LOP03611, LOP07256 | 13 |
aNo NSY hortologue was identified; clusters encode for a ortholog of human TGF-beta activated kinase MAPKKK7
bIn C. elegans the Gqα signaling component EGL-30 is also expressed in the neuron and can modulate intestinal immunity