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Effect of mTOR-siRNA on cell proliferation. NSCLC cells were seeded onto 6-well plates at a density of 10,000 cells/wells and treated with the transfection reagent alone or with mTOR-siRNA. After 48 hours of incubation, 2.5 mL of 10% media was added to each well, and the cells were incubated for an additional 48 hours. Cell proliferation was measured by counting the number of cells from five independent high-powered fields (x 200) (*p = 0.034).
