Figure 5.

Effect of mTOR-siRNA on cell migration. NSCLC cell migration was examined using a Boyden microchemotaxis chamber. NSCLC cells were treated with the transfection reagent alone (control) or with mTOR-siRNA, plated in the upper compartment and allowed to migrate through 8-μm-sized pores. After 6 hours, migrated cells were fixed and stained with hematoxylin and eosin. Chemotaxis in each well was assessed by counting the number of cells from five independent high-powered fields (x 200) (*p = 0.0001).