Table 5.
Serum concentrations of soluble TNF receptors and flow cytometry analysis of surface expression of TNFα-pathway proteins on peripheral blood cells stratified by group-specific median serum concentration of TNFR2
| Characteristic | Group Stratification (TNFR2 Median)a | P Valueb | |
|---|---|---|---|
| Low (n=17) | High (n=17) | ||
| Serum sTNFR2 (pg/ml) | 2442±260 | 4079±1098 | By design |
| Serum sTNFR1 (pg/ml) | 1310±276 | 2291±773 | <0.001 |
| Leukocyte counts (×109/L) | 6.6±1.7 | 6.4±1.9 | 0.63 |
| neutrophils | 4.4±2.0 | 4.2±1.3 | 0.81 |
| lymphocytes | 2.8±4.5 | 1.5±0.6 | 0.25 |
| monocytes | 0.4±0.1 | 0.5±0.2 | 0.07 |
| Surface expression of TNFα-pathway proteinsc | |||
| TNFα protein on | |||
| neutrophils | 24±21 | 17±25 | 0.24 |
| T lymphocytes, CD3+ | 1.4±1.8 | 1.5±1.8 | 0.87 |
| monocytes | 24±17 | 25±31 | 0.76 |
| TNFR1 protein on | |||
| neutrophils | 105±58 | 87±82 | 0.24 |
| T lymphocytes, CD3+ | 2.0±1.7 | 1.5±2.8 | 0.50 |
| monocytes | 135±71 | 121±104 | 0.37 |
| TNFR2 protein on | |||
| neutrophils | 27±10 | 24±7 | 0.34 |
| T lymphocytes, CD3+ | 9±6 | 9±4 | 0.97 |
| monocytes | 122±32 | 115±31 | 0.48 |
| ADAM17 protein on | |||
| neutrophils | 77±6.2 | 74±6.6 | 0.15 |
| T lymphocytes, CD3+ | 3.1±1.1 | 3.3±1.3 | 0.62 |
| monocytes | 141±13 | 133±17 | 0.12 |
Data are the mean ± SD.
a
Low and high denote strata below and above median TNFR2 in each group (2769 pg/ml), accordingly.
b
P value for t test on log-transformed values.
c
Data are mean fluorescence intensities, the differences between the intensities for the surface marker and the isotype control.