Table 1.

Mössbauer and spin Hamiltonian parameters of the high- and low-spin ferric hemes in native and Ca²⁺-depleted MauG

	Native MauG Hemes			Ca2+-depleted MauG hemes
	HSa	LSa	LSb	LS*
Dc (cm-1)	10
E/D c	0
Δ/λc			2.95	3.43
V/Δc			0.68	0.55
g x	5.57	1.87	1.52	1.52
g y	1.99	2.19	2.32	2.27
g z	1.99	2.54	2.89	2.97
Ax/gn/βn (T)	–18.0	–35.0	–38.7	–32.0
Ay/gnβn (T)	–18.0	9.30	22.6	15.0
Az/gnβn (T)	–18.0	29.4	50.6	50.0
ΔEQ (mm/s)	2.0	2.09	2.44d	2.17d
δ (mm/s)	0.50	0.25	0.33	0.28
η e	0.4	–3.0	–3.0	–2.0

^aThe His-Tyr heme

^bLow-spin species derived from freezing of the HS heme

^cΔ and V are the axial and rhombic crystal field parameters and λ is the spin-orbit coupling constant; D is zero-field splitting; E/D is rhombicity parameter; A_n/g_nβ_n is the saturation field

^dvalue at 180 K

^eη = (V_xx – V_yy)/ V_zz, where V_ii values are the principle components of the electric field gradient tensor.