Fig. 3.

Molecular marker expression in WT and 2-OST morphant embryos. (A-P) Wild-type embryos were injected with 3 ng of either control MO or 2-OST MO, fixed at 8 hpf, and processed for whole-mount in situ hybridization. Expression patterns of mesoderm markers (A-F), mesendoderm markers (G-J), a DFC and endoderm marker (K,L), an ectoderm marker (M,N) and a YSN marker (O,P) were analyzed. The presence and organization of these markers in both the WT and morphant embryos suggest that 2-OST morphants were establishing the primary germ layers and axes. Furthermore, the presence of sox17 and shh, which are expressed later in development, indicate that the 2-OST morphants were not severely delayed in development.