Fig. 5.

2-OST modulates active β-catenin protein, but not transcriptional responses. (A) Western blot for antibody against the active form of β-catenin at 4 hpf reveals that levels of active β-catenin were decreased in 2-OST morphants compared with wild type and control morphant (^*P<0.05) but total levels of active β-catenin were restored when 2-OST MO was co-injected with the stabilized β-catenin RNA. Levels were normalized to WT relative to levels of α-tubulin for each sample (two embryos/lane/sample, n=4 experiments). (B) qPCR for EGFP transcript in TOP-dGFP fish as well as axin2 and dkk1b, which encode Wnt transcriptional targets, normalized to WT levels, analyzed using ΔΔcT method. y-axis indicates fold difference (combined data from n=8 series of experiments; ^*P<0.05 from WT and control MO, ^**P<0.05 from WT, control MO, and 2-OST MO). Error bars represent s.d.