Fig 3.

Promoter activities of IGr sequences from various ves loci. B. bovis C9.1-parasitized erythrocytes were cotransfected with luciferase constructs containing the IGr and apposing gene sequences to the 3′ end of intron 1 from various ves loci. p1E10 and pS621 IGr sequences are derived from ves loci that are nontranscribed in the B. bovis C9.1 clonal line (13). Note that the S621 construct contains such sequences from a ves1α-ves1α pair. In this and subsequent figures, arrows above the LAT schematic drawing indicate the portion(s) of the locus (or comparable regions of other loci) included in luciferase constructs. Unless indicated otherwise, these sequences were included as putative promoters, and arrowheads indicate the 3′ end of each sequence adjacent to the luciferase gene. Asterisks indicate samples with values statistically significantly different from those determined with the pLuc-T3 promoterless control (P ≤ 0.05).