Fig 6.

ves1α and ves1β introns have no apparent promoter-like activity. ves1α introns 1 and 2 and ves1β intron 1 were tested directly for promoter activity by insertion into the pLuc-T3 construct to drive luciferase expression. None of these sequences demonstrated promoter activity significantly greater than that of the pLuc-T3 promoterless control. This experiment was repeated two times, using B. bovis CE11-parasitized erythrocytes. The asterisk indicates a statistically significant difference from pLuc-T3 at P ≤ 0.05; the plus signs indicate those samples whose results are statistically significantly different from those of pLAT_Vα5-βE1 at P ≤ 0.05. No other pairwise comparisons revealed significant differences.