Fig 3.

The ΔaspB mutant shows clumped nuclei. (A) Wild-type nuclei positioned along hyphae. (B) The ΔaspB mutant results in clumped nuclei, particularly near branches. (C) The ΔaspB mutant shows clumped nuclei. The ΔaspB mutant showed more nuclei in 15 μm around the most basal branch than did the wild-type strain. To delineate the area for nuclear counts, the most basal branch was identified; 7.5 μm from the center of the branch to the left and right of the branch was measured (total area = 15 μm; n = 200). Spores were incubated for 14 to 16 h at 30°C, and nuclei were stained with Hoechst 33258. Scale bar, 5 μm.