Fig 4.

AspB forms rings, dots, bars, and filaments. The AspB-GFP strain was incubated at 30°C for 4 to 5 h to view isotropic growth, 6 to 7 h to view early germ tube emergence, 9 to 11 h to view hyphal elongation and septation, and 12 to 16 h to view branching. (A) AspB forms bars (*), rings (<), and dots (arrowhead) in dormant and germinating spores. The inset shows an enlarged view of a ring (scale bar, 0.5 μm). (B to D) AspB forms “X's” (#) in addition to bars, rings, and dots as conidia swell. The inset shows an enlarged view of an “X” (scale bar, 0.75 μm). AspB forms caps and collars as the germ tube emerges, bars that localize to conidia and filaments (arrows) that localize to tips. (E to G) AspB forms bars that localize subapically and filaments that localize to tips as the hypha extends. Cytoplasmic GFP fluorescence is excluded from nuclei (N). (H) AspB forms rings at septa. (I to R) AspB forms filaments in branching compartments, caps as the branch emerges, and dots at the tips of branches. Filaments localize to newly formed branches. (S) Filaments and bars localize to longer branches. (T) Top view of a branch cap. Scale bar, 5 μm.