Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotypea | Source or referenceb |
|---|---|---|
| pFNO3 | ga5-gfp AfpyrG kan | FGSC (67) |
| A1145 (TN02A7) | pyrG89; pyroA4; nkuA::argB; riboB2 | FGSC (67) |
| A1147 (TNO2A25) | pyrG89; argB2; pabaB22nku::argB; riboB2 | FGSC (67) |
| A850 | biA1; _argB::trpC_B; methG1; veA1; trpC801 | FGSC |
| A773 | pyrG89; wA3; pyroA4 | FGSC |
| ARL115 | pyrG89 aspB::aspB-gfp-AfpyrG; argB2; pabaB22; nku::argB; riboB2 | This study |
| ARL144 | pyrG89 aspB::AfpyrG; pyroA4; nkuA::argB; riboB2 | This study |
| ARL148 | pyrG89 aspD::AfpyrG; pyroA4; nkuA::argB; riboB2 | This study |
| ARL157 | aspC::AfpyrG; pyrG89; pyroA4 | 38 |
| ARL162 | aspC::AfpyrG; aspA::argB2; pyrG89; pyroA4; biA1_argB::trpC_B; veA1; trpC801 | 38 |
| AYR1 | pyrG89 aspB::AfpyrG; pyroA4; riboB2 | This study |
| AYR6 | pyrG89 aspB::aspB-gfp-AfpyrG; argB2 | This study |
| AYR10 | aspB::aspB-gfp-AfpyrG\\ | This study |
| AYR20 | aspB::aspB-gfp-AfpyrG; aspA::argB2 | This study |
| AYR21 | aspB::aspB-gfp-AfpyrG; aspA::argB2 | This study |
| AYR22 | aspB::aspB-gfp-AfpyrG; aspA::argB2 | This study |
| AYR23 | aspB::aspB-gfp-AfpyrG; aspC::AfpyrG | This study |
| AYR24 | aspB::aspB-gfp-AfpyrG; aspC::AfpyrG | This study |
| AYR25 | aspB::aspB-gfp-AfpyrG; aspC::AfpyrG; aspA::argB2 | This study |
| AYR26 | aspB::aspB-gfp-AfpyrG; aspE::AfpyrG | This study |
| AYR27 | aspB::aspB-gfp-AfpyrG; aspE::AfpyrG | This study |
| AYR32 | aspB::AfpyrG; pyroA4; argB2 | This study |
| AYR35 | aspB::aspB-gfp-AfpyrG; pyroA4 | This study |
| AYR43 | aspD::AfpyrG | This study |
| AYR45 | aspB::aspB-gfp-AfpyrG; nkuA::argB; aspD::Afpyr | This study |
| AYR64 | aspB::aspB-gfp-AfpyrG; pyroA4; aspD::pyrGAf | This study |
| AYR65 | aspB::aspB-gfp-AfpyrG; pyroA4; aspD::pyrGAf | This study |
| ASH26 | aspA::argB2 pyrG89 wa3argB::trpC_B methG1 pyroA4 | 38 |
| ASH41 | aspE::AfpyrG; riboB2 | This study |
a
The symbol “\\” indicates haploids fused to make a diploid strain.
b
FGSC, Fungal Genetics Stock Center, Department of Microbiology, University of Kansas Medical Center (Kansas City, KS).