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(A) Domain structure of Um10417. Deleted regions in the respective strains and primer binding sites for the verification of transcript abundance via RT-PCR are indicated. (B) RT-PCR analysis of Um10417 deletion strains. The um10417-specific primer pairs correspond to the ones shown in panel A. The glyceraldehyde-3-phosphate dehydrogenase gene (GapDH) was used as a positive control. Contamination by genomic DNA was excluded beforehand with genomic DNA-specific primers.
