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Growth of U. maydis sporidia on minimal medium plates supplemented with 0.3% (wt/vol) ammonium (AMM) (A) or 0.3% (wt/vol) nitrate (NMM) (B). Dilution series (OD600 of 1 to 10−4 in 10−1 steps from left to right) of SG200, Δnit2, ΔN-Nit2, and Δnit2 complemented with Nit2 (Δnit2-Nit2) were plated onto the respective medium, and growth was assessed after incubation at 28°C for 1 day.
