Fig 6.

Pathogenicity of U. maydis strains on maize leaves. (A) Maize leaves 10 days postinfection with SG200, Δnit2, or Δnit2 complemented with a genomic promoter-gene fragment of nit2. (B) Maize leaves 8 days postinfection with SG200 or Δton1. Two representative leaves per strain are shown. In total, between 12 and 20 infected seedlings per strain were analyzed. (C) Disease index for SG200, Δnit2, or Δnit2-Nit2 scored at 10 dpi. Maize seedlings were infected by injecting sporidia suspensions (OD₆₀₀ of 1.0) of the respective strain into the leaf canal with a syringe. The incidence of the indicated symptoms was scored as described in Materials and Methods (n = 12 to 20). Results for one representative out of four experiments are displayed.