Table 2.
Summary of observed growth of SG200, Δnit2, and Δnit2-Nit2 sporidia on minimal medium plates supplemented with a single nitrogen sourcea
Supplement | Growth observed for: |
||
---|---|---|---|
SG200 | Δnit2 | Δnit2-Nit2 | |
Ammonium | + | + | + |
Nitrate | + | −− | + |
Ala | + | − | + |
Arg | + | − | + |
Asn | + | + | + |
Asp | + | − | + |
Citrullin | + | −− | + |
Cys | + | −− | + |
Gln | + | + | + |
Glu | + | + | + |
Gly | + | −− | + |
His | + | −− | + |
Ile | + | −− | + |
Leu | + | −− | + |
Lys | + | − | + |
Met | + | −− | + |
Ornithine | + | − | + |
Phe | + | −− | + |
Pro | + | − | + |
Ser | + | −− | + |
Thr | + | −− | + |
Trp | + | − | + |
Tyr | + | +/− | + |
Uracil | + | − | + |
Val | + | −− | + |
Adenine | + | −− | + |
Cytosine | + | −− | + |
Guanine | + | − | + |
Thymine | + | −− | + |
Putrescine | + | + | + |
Spermidine | + | − | + |
Spermine | −− | −− | −− |
Proline-chlorateb | − | + | − |
Sporidial dilution series of each genotype were plated, and growth properties were rated 1 day after plating as normal growth (+), reduced growth (+/−), strongly reduced growth (−), and no growth (−−). ND, not determined. Resistance to chlorate was assessed 7 days after plating in the presence of proline.
Growth characteristics in the chlorate assay are not compared to growth on media without chlorate.