Table 3.
Verification of transcript accumulation of selected genes by qRT-PCRa
| Gene name | Annotation | Change in transcript accumulation by strain and medium |
Category | |||
|---|---|---|---|---|---|---|
| SG200 |
Δnit2 |
|||||
| −N vs AMM | NMM vs AMM | −N vs AMM | NMM vs AMM | |||
| um01756.2 | Purine transporter | 12 ± 5 | 1.7 ± 0.2 | 0.7 ± 0.1 | 0.45 ± 0.01 | Nit2-dependent induction |
| um03690 | Purine permease | 671 ± 161 | 39 ± 7 | 2.9 ± 0.5 | 1.9 ± 0.2 | Nit2-dependent induction |
| um10005 | Gal4-TF (ton1) | 12 ± 2 | 3.1 ± 0.4 | 1.6 ± 0.5 | 1.9 ± 0.2 | Nit2-dependent induction |
| um04577 | Urea permease | 1,236 ± 530* | 702 ± 114* | 7 ± 2 | 12 ± 5 | Nit2-dependent induction |
| um03847 | nar1 | 131 ± 32 | 71 ± 8 | 13 ± 3 | 23 ± 3 | Partial Nit2-dependent induction |
| um11104 | nir1 | 2,484 ± 787* | 2,013 ± 393* | 154 ± 60 | 294 ± 90 | Partial Nit2-dependent induction |
| um11105 | nrt | 3,281 ± 863* | 3,604 ± 703* | 232 ± 70 | 559 ± 16 | Partial Nit2-dependent induction |
| um05889 | ump2 | 996 ± 73* | 423 ± 28 | 177 ± 21 | 152 ± 24 | Partial Nit2-dependent induction |
| um02625 | Urea permease | 0.0027 ± 0.0004 | 10 ± 2 | 0.021 ± 0.007 | 0.045 ± 0.008 | Nit2-dependent induction on NMM only |
| um00477 | mct1 | 28 ± 8 | 1.2 ± 0.8 | 153 ± 42 | 60 ± 2 | Nit2-dependent repression |
| um04060 | dic1 | 65 ± 10 | 17 ± 3 | 52 ± 12 | 50 ± 9 | Nit2 dependent repression on NMM |
| um06253 | Urea permease | NR | NR | NR | NR | Not regulated |
a
SG200 and Δnit2 sporidia were transferred to nitrate minimal medium (NMM), nitrogen starvation (−N), or ammonium minimal medium (AMM) and harvested 2 h after transfer. Shown is the absolute fold change and standard errors of the respective genes in −N or NMM compared to levels for the control determined from three experimental replicates with three technical replicates each. Values marked with an asterisk show strong induction due to a basically absent transcript on AMM and have to be considered qualitative, not quantitative.