Table 1.
Putative TOC and TIC components in the Bigelowiella natans genomea
| Component | P. sativum accession no. | B. natans accession no.b | No. of aa | Presence of: |
TPL net charge (no. of aa) | E value | |
|---|---|---|---|---|---|---|---|
| SP sequence (probability score) | TPL sequence (probability score) | ||||||
| TOC34 | Q41009 | — | |||||
| TOC64 | AAF62870 | (JGI 89506) | 784 | Yes (0.998) | No (0.493) | 4 (50) | 7.00E−20 |
| TOC75 | Q43715 | ABA27321(Nm) | 818 | No | No (0.427) | −2.9 (50) | 9.00E−24 |
| OMP85 | Q43715 | JGI 79166 | 899 | Yes (0.786) | No (0.435) | 10 (50) | 0.002 |
| TOC159 | AF75761 | — | |||||
| TIC20 | AAC64607 | ABA27416 (Nm) | 207 | No | No (0.458) | 9 (50) | 5.00E−5 |
| JGI 115241 | 322 | Yes (1.000) | Yes (0.549) | 3.1 (32) | 1.00E−5 | ||
| TIC21 | ABG00264 | JGI 66755 | 272 | Yes (1.000) | Yes (0.518) | 7.1 (36) | 2.00E−4 |
| TIC22 | AAC64606 | JGI 127449 | 341 | Yes (0.996) | Yes (0.530) | 8 (32) | 0.001 |
| JGI 66754 | 330 | Yes (0.948) | Yes (0.538) | 9.1 (53) | 0.15 | ||
| TIC32 | AAS38575 | JGI 91380 | 527 | Yes (0.329) | Yes (0.521) | 0 (9) | 3.00E−32 |
| TIC40 | CAB50925 | JGI 82201 | 569 | Yes (0.574) | Yes (0.556) | 11.2 (58) | 2.00E−22 |
| TIC55 | CAA04157 | JGI 77479 | 648 | Yes (0.600) | Yes (0.514) | 6.2 (26) | 7.00E−59 |
| TIC62 | CAC87810 | JGI 86815 | 339 | Yes (0.835) | Yes (0.560) | 12.3 (53) | 3.00E−46 |
| TIC110 | CAA92823 | — | |||||
a
The GenBank accession or JGI protein identification number of each TOC/TIC component is recorded. “SP sequence” indicates the result of signal peptide prediction by the HMM method of SignalP, and the probability scores are shown in parentheses. “TPL sequence” is the result of transit peptide prediction by ChloroP. “TPL net charge” shows the net charge of the predicted TPL sequence, with the predicted length of the TPL sequence in parentheses (the N-terminal 50 aa of TOC64, TOC75, and TIC20 were used for the net charge calculation, since their TPL regions could not be predicted by ChloroP). The BLASTP E values against P. sativum TOC/TIC components are in the rightmost column.