Fig 3.

Influence of Ulp1-Gal4AD-YFP on localization of Gal3p-CFP and Gal80p-CFP. Cells producing a carboxyl-terminally CFP-tagged version of either Gal80p (A to C) or Gal3p (D to F) also produced a fusion protein composed of the first 150 amino acids of Ulp1p, amino acids 768 to 881 of Gal4p, and YFP (Ulp1-Gal4AD-YFP). Cells were grown in 2% raffinose (A and D) or washed from this medium and resuspended in 1% raffinose and 1% galactose (B, C, E, and F). For cells in the presence of galactose, images were collected either 10 min after the addition of the fresh medium (B and E) or after 120 min (C and F). The merged panels are overlays of the YFP and CFP images. Bars = 5 μm.