Fig 1.
Substrate specificity of LC-cutinase*. The enzymatic activity was determined at 30°C in 25 mM Tris-HCl (pH 8.0) containing 10% acetonitrile using pNP-acetate (C2), pNP-butyrate (C4), pNP-hexanoate (C6), pNP-caprylate (C8), pNP-laurate (C12), pNP-myristate (C14), pNP-palmitate (C16), or pNP-stearate (C18) as a substrate. The specific activities relative to that determined for hydrolysis of pNP-butyrate are shown. The experiment was carried out at least twice, and the average values are shown together with error bars.