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Degradation of PCL film by LC-cutinase*. A PCL film (20–30 mg) was incubated at 50°C for 6 h in 1 ml of 500 mM Tris-HCl (pH 8.0) containing 5 μg of LC-cutinase* (LCC), or 50 μg of B. cepacia lipase (Bc-Lip) or C. rugosa lipase (Cr-Lip), and its weight loss after incubation was determined. The experiment was carried out at least twice and the average values are shown, together with error bars.
