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SDS-PAGE for expression of recombinant cellobiose phosphorylase from E. coli strains with and without LacY mutation. Cell pellets were harvested after 24 h of cultivation, and protein samples were prepared according to the manufacturer's manual (Bio-Rad). Each lane was loaded with 20 μg proteins. Lanes: M, Mw marker; A, KO11 No-CepA (KO11/pQE80L); B, KO11 CepA; C, KO11 DelLacY CepA; D, KO11 DelLacY CepA-LacY.
