Table 2.
Microarray analysis of gene expression in C. jejuni NCTC11168 in brucella broth with 0.6% TSP compared to brucella broth (pH 7)
| Systematic | Relative gene expressiona at: |
Product | Additional functional description | |||
|---|---|---|---|---|---|---|
| 5 min |
30 min |
|||||
| Fold change | P | Fold change | P | |||
| Cj0008 | 2.2 | 0.000 | 1.8 | 0.090 | Hypothetical protein Cj0008 | |
| Cj0039c | 1.3 | 0.024 | 1.8 | 0.000 | GTP-binding protein typA homolog | |
| Cj0044c | –1.3 | 0.026 | –2.0 | 0.000 | Hypothetical protein Cj0044c | |
| Cj0045c | 1.0 | 0.859 | –1.7 | 0.000 | Putative iron-binding protein | |
| Cj0176c | 2.5 | 0.001 | 3.2 | 0.000 | Putative lipoprotein | In operon with Cj0173-Cj0175c, which is involved in scavenging iron from lactoferrin and transferrinb |
| Cj0403 | 1.3 | 0.045 | 1.5 | 0.001 | Hypothetical protein Cj0403 | |
| Cj0608 | 2.4 | 0.000 | 1.4 | 0.004 | Putative outer membrane protein | |
| Cj0717 | 1.3 | 0.115 | 1.7 | 0.000 | Hypothetical protein Cj0717 | |
| Cj0770c | 1.1 | 0.298 | 2.0 | 0.000 | Putative periplasmic protein | Contains a conserved domain characteristic of the periplasmic component of an ABC transporterc |
| Cj0771c | 1.3 | 0.089 | 1.8 | 0.000 | Putative periplasmic protein | |
| Cj1000 | 1.8 | 0.018 | 1.7 | 0.000 | Putative transcriptional regulator (LysR family) | |
| Cj1241 | –1.5 | 0.001 | –1.6 | 0.000 | Putative transmembrane transport protein | |
| Cj1436c | –1.4 | 0.025 | –1.6 | 0.000 | Putative aminotransferase | |
| Cj1467 | –1.3 | 0.098 | –2.0 | 0.001 | Hypothetical protein Cj1467 | |
| Cj1471c | –1.7 | 0.006 | –2.3 | 0.001 | Putative type II protein secretion system E protein | Essential for DNA uptake and natural transformationd |
| Cj1483c | –1.4 | 0.326 | –1.8 | 0.000 | Putative lipoprotein | |
| Cj1533c | 1.6 | 0.002 | 1.8 | 0.000 | Putative helix-turn-helix containing protein | |
| Cj1646 | –1.5 | 0.070 | –2.0 | 0.000 | Putative ABC transport system permease protein | |
a
Relative gene expression is presented as the mean ratio of fluorescence intensity of TSP exposed cells to that of pH 7-exposed cells. Genes with the fold change shown in boldface indicate significant differential expression (Student t test with a P value of <0.05 adjusted with the Benjamini-Hochberg false-discovery-rate multiple testing correction). All P values were calculated using the Student t test.
c
Information revealed from bioinformatic analysis.
d
Function described previously (38).