Table 2.
Anaerobic and aerobic growth tests of denitrifying strain pMbN1 with different aromatic and nonaromatic compoundsa
| Compound tested (concn)b | Growthc |
|
|---|---|---|
| Anaerobic | Aerobic | |
| Aromatic compounds | ||
| 4-Methylbenzoate (1, 2.5) | + | + |
| 3-Methylbenzoate (1, 2) | + | − |
| Benzoate (1, 4) | + | + |
| 2-Aminobenzoate (1, 2) | + | − |
| 4-Hydroxybenzoate (1, 4) | + | − |
| Phenylacetate (1, 4) | + | − |
| Cinnamate (2) | + | + |
| Hydrocinnamate (2) | + | + |
| p-Coumarate (2) | + | + |
| Phenol (0.5, 2) | + | − |
| p-Cresol (0.5, 2) | + | − |
| Benzyl alcohol (0.5, 2) | + | − |
| Benzaldehyde (0.5, 2) | + | − |
| Nonaromatic compounds | ||
| Acetate (1, 5) | + | + |
| Propionate (1, 4) | + | + |
| Butyrate (1, 4) | + | + |
| Isobutyrate (1, 4) | − | + |
| Adipate (1, 5) | + | + |
| Lactate (5, 10) | + | + |
| Malate (1, 5) | + | + |
| Pyruvate (1, 5) | + | + |
| Succinate (1, 5) | + | + |
| Ethanol (1, 5) | + | + |
| Complex media | ||
| Yeast extract (0.5% [wt/vol]) | + | + |
| Peptone (0.5% [wt/vol]) | − | − |
| Casamino acids (0.5, 2% [wt/vol]) | − | − |
Further compounds tested but not utilized by strain pMbN1 under nitrate-reducing or oxic conditions are as follows (concentrations given in percent volume/volume refer to dilutions of poorly water soluble compounds in heptamethylnonane as inert carrier phase): toluene (2%), 4-ethyltoluene (2%), ethylbenzene (2%), propylbenzene (2%), m-xylene (2%), p-xylene (1%), 2-methylbenzoate (1, 2 mM), 4-ethylbenzoate (1, 2 mM), 4-propylbenzoate (0.5, 2 mM), 4-isopropylbenzoate (1, 2 mM), 4-butylbenzoate (0.5, 2 mM), 4-tert-butylbenzoate (0.5, 2 mM), terephthalate (0.5, 2 mM), resorcinol (1 mM), o-cresol (0.5, 2 mM), m-cresol (0.5, 2 mM), 1-phenylpropanol (0.5, 2 mM), (R)-1-phenylethanol (0.5, 2 mM), (S)-1-phenylethanol (0.5, 2 mM), acetophenone (1%), propiophenone (1%), p-cymene (2, 5%), α-phellandrene (1, 2%), α-terpinene (1, 2%), limonene (1, 2%), cyclohexane carboxylate (0.5, 2 mM), cyclohexanol (1, 2%), cyclohexane-1,2-diol (1, 5 mM), n-hexane (2%), glucose (1, 5 mM), fructose (1, 5 mM), all 20 amino acids (0.5, 2 mM), ascorbate (4 mM), formate (10, 20 mM), acetone (0.5, 2 mM).
Each compound was tested at the concentrations shown in parentheses and given in mM unless otherwise indicated.
+, optical density at 660 nm of >0.1; −, no growth.