Three experimental conditions, as previously reported (22), were used to examine the gene transcription of DET1576 and DET0138 in this study. ♦ represents cultures incubated with ammonium, ● represents cultures incubated without ammonium, and ■ represents cultures incubated initially without ammonium but which later received a dose of ammonium at the time indicated by the arrow. (A) Dechlorination profile of VC production. (B) Growth characteristics. (C) DET1576 transcription (this study). (D) DET0138 transcription (this study). Transcription data are averages of triplicate RT-qPCR data from each biological duplicate, and error bars represent 1 standard deviation. (Panels A and B reprinted from reference 22.)