Table 2.
Effect of headspace oxygen concentration on generation time, final cell yield, and final medium pH of Diplosphaera colitermitum strain TAV2a
| Headspace O2, % (no. of replicates) | Mean ± SD |
||
|---|---|---|---|
| Generation time, h | Final cell yield, cells/ml | Final pH | |
| 0 (3) | NGb | ||
| 2 (6) | 7.7 ± 0.7 | 2.5 × 109 ± 7.4 × 107 | 6.0 ± 0.1 |
| 4 (6) | 8.3 ± 0.4 | 3.6 × 109 ± 2.7 × 108 | 6.3 ± 0.1 |
| 8 (6) | 7.7 ± 0.3 | 3.3 × 109 ± 2.3 × 108 | 6.3 ± 0.1 |
| 12 (6) | 9.2 ± 0.6 | 3.7 × 109 ± 5.3 × 108 | 6.2 ± 0.1 |
| 16 (6) | 8.9 ± 0.8 | 3.2 × 109 ± 2.3 × 108 | 6.2 ± 0.1 |
| 20 (6) | 11.7 ± 1.4 | 2.6 × 109 ± 1.2 × 108 | 6.2 ± 0.1 |
| Air (3) | 14.0 ± 0 | 3.7 × 109 ± 6.0 × 107 | 6.6 ± 0.1 |
a
Growth at O2 concentrations of 0 to 20% was measured in enclosed anaerobe tubes without replacement of the atmosphere. Growth in air was measured in anaerobe tubes with caps that allowed continual influx of atmosphere.
b
NG, no growth.