The results of plaque assays were used to calculate the total recovery rates. (a) Total recovery rates for D29 aerosols as a function of spray liquids; (b) total recovery rates of four sample liquids for D29 aerosols; (c) effect of relative humidity on the recovery rates of the D29 aerosols in the test chamber after it decayed for 15 min (■) and 30 min (●); (d) effect of storage temperature and storage time on phage D29 culturability (■, 4°C, the sample medium is nutrient broth; ●, 25°C, the sample medium is nutrient broth; ▲, 4°C, the sample medium is SM buffer; ▼, 25°C, the sample medium is SM buffer). Each column represents the mean value of at least three experiments, and the error bars represent the standard deviation.