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SDS-PAGE of RAC affinity pulldown for cell wall proteins eluted from B. subtilis strains. Lane 1, cell-bound LMC from B. subtilis(pNWP43N-LMC) cells; lane 2, cell-bound unifunctional minicellulosome from B. subtilis(pNWP43N-LMC) cells premixed with BsCel5′; lane 3, cell-bound trifunctional minicellulosome from B. subtilis(pNWP43N-LMC) cells premixed with BsCel5′, CtCel9, and CpCel48.
