Table 2.

Metabolic activities of 11 Brucella microti strains^a

Substrate or reaction	Abbreviation	Substrate class	Activity of the following Brucella microti strain:
			CCM 4915T	CCM 4916	IBM 284	IBM 257	FH 2208	FK 21908	FW 70608	F 303 Mi	BMS 10	BMS 17	BMS 20
Leu-pNA	LNA	Aminopeptidases with pNA	+	+	++	+	+	++	+	+	+	++	++
Ac-Gly-Lys-β	AcGK	Aminopeptidases with βNA	+	+	−	−	−	−	−	−	−	−	−
Ala-Phe-Pro-Ala-β	AFPA	Aminopeptidases with βNA	+	++	+	+	+	+	+	+	+	+	+
Asn-β	N	Aminopeptidases with βNA	−	+	−	−	−	−	−	−	−	−	−
Ac-Lys-Ala-β	AcKA	Aminopeptidases with βNA	+	+	−	−	−	−	−	−	−	−	−
d-Ala-d-Ala-β	dAdA	Aminopeptidases with βNA	++	+	+	+	+	+	++	+	+	+	+
Val-Tyr-Ser-β	VTS	Aminopeptidases with βNA	++	+	+	−	−	−	+	−	−	+	−
p-Nitrophenyl-α-d-maltoside	aMAL7	Glucosidases	+	++	+	+	+	+	+	+	+	+	+
p-Nitrophenyl-α-d-xylopyranoside	aXYL7	Glucosidases	+	++	+	+	+	+	+	+	+	+	+
d(−)-Ribose	d-RIB	Monosaccharides	+	++	++	++	++	+	++	++	++	+	++
d(−)-Arabinose	d-ARA	Monosaccharides	++	−	++	−	++	++	++	++	++	++	++
d(+)-Xylose	d-XYL	Monosaccharides	+	++	+	++	++	+	++	++	++	+	++
d-Threitol	d-TOL	Sugar derivates	++	++	+	++	++	++	++	+	++	+	++
dl-Lactic acid	dlLac	Organic acids	+	++	+	++	++	+	++	+	+	+	+
l-Asparagine	l-Asn	Amino acids	+	++	+	++	++	++	++	++	++	+	++
l-Glutamic acid	l-Glu	Amino acids	+	++	++	++	++	++	++	++	++	++	++
d-Alanine	d-Ala	Amino acids	++	++	++	++	++	++	++	++	++	−	−
Propionic acid	Propn	Organic acids	−	−	++	++	++	++	++	++	++	−	++
dl-β-Hydroxybutyric acid	βHBut	Organic acids	++	−	++	++	++	++	++	−	++	−	++
Nα-Acetyl-l-arginine	AcArg	Amino acid derivates	++	++	−	++	++	−	++	−	−	−	−
Hippuryl-Arg	HipArg	Peptides	++	++	−	++	++	−	++	−	−	−	−
Glycine-free base	Gly	Amino acids	−	++	++	++	++	++	++	++	++	++	−
Nitrite reduction	NTI	Classical reactions	−	+	+	+	+	++	++	+	++	++	+
Voges-Proskauer	VP	Classical reactions	++	+	+	+	++	++	++	++	++	+	+
Nitrate reduction	NTA	Classical reactions	−	+	+	+	+	++	++	+	++	++	+
Glu(pNA)-OH	ENAOH	Aminopeptidases with pNA	−	−	−	−	−	−	−	−	−	−	−
Pyr-pNA	PYRNA	Aminopeptidases with pNA	−	−	−	−	−	−	−	−	−	−	−
H-Hydroxyproline-βNA	HP	Aminopeptidases with βNA	++	++	++	++	++	++	++	++	++	++	++

^aA representative selection of the 93 substances tested in the Brucella-specific Micronaut assay has been merged (3). The quality of each biochemical reaction is presented as follows: −, no metabolic activity; +, moderate metabolic activity; ++, strong metabolic activity. Brucella-specific traits are shown in boldface, and clearly distinctive features of the isolates are shaded.