Table 1.
Strains and plasmids used in this study
| Strain, derivative, or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| C. acetobutylicum ATCC 824 | Wild type | KBRCb |
| C. acetobutylicum PJC4BK | buk::erm | 9 |
| E. coli TOP10 | Invitrogen | |
| Derivatives and plasmids | ||
| pAN1 | Cmr, ϕ3T I gene, p15A origin | 24 |
| pIMP1 | Apr MLSrrepL, ColE1 origin | 25 |
| pIMP2 | pIMP1 derivative with a substituted multiple cloning site | This study |
| pUC18 | Apr, ColE1 origin | 27 |
| pUCadcT | pUC18 derivative with adc terminator insertion | This study |
| pUCadcPT | pUCadcT derivative with adc promoter insertion | This study |
| pSADH1 | pUCadcPT derivative with adhB-593 (adc promoter) | This study |
| pACT | pIMP2 derivative with adc ctfAB (adc promoter) insertion | This study |
| pIPA1 | pIMP2 derivative containing adhB-593 (adc promoter) | This study |
| pIPA3 | pACT derivative containing adhB-593 (adc promoter) | This study |
| pIMP30 | pIMP2 derivative, AprrepL, ColE1 origin | This study |
| pIMP3 | pIMP30 derivative, Cmr | This study |
| pACT-Cm | pIMP3 derivative with adc ctfAB (adc promoter) insertion | This study |
| pIPA3-Cm2 | pACT-Cm derivative containing adhB-593 (adc promoter) | This study |
a
Cmr, chloramphenicol/thiamphenicol resistance; MLSr, erythromycin resistance; adc, acetoacetate decarboxylase gene from C. acetobutylicum; ctfAB, CoA transferase genes from C. acetobutylicum; adhB-593, a primary/secondary alcohol dehydrogenase gene from C. beijerinckii NRRL B-593; Apr, ampicillin resistance; repL, pIM13g-positive origin of replication.
b
KBRC, Korea Research Institute of Bioscience and Biotechnology Biological Resource Center, Daejeon, Republic of Korea.