Table 3.
Flask cultures of the engineered and control strains
Strain | Titer for characteristic or product (g/liter)a |
||||||
---|---|---|---|---|---|---|---|
Glucose utilized | Acetone | Isopropanol | Butanol | Ethanol | Acetic acid | Butyric acid | |
Wild-type | 40.0 ± 1.0 | 3.8 ± 0.2 | ND | 8.2 ± 0.3 | 0.8 ± 0.0 | 1.3 ± 0.1 | 0.6 ± 0.1 |
824(pIPA1) | 38.8 ± 1.4 | 0.1 ± 0.0 | 3.1 ± 0.2 | 7.4 ± 0.2 | 0.8 ± 0.1 | 1.4 ± 0.2 | 0.9 ± 0.1 |
824(pACT) | 47.7 ± 2.0 | 5.1 ± 0.2 | ND | 8.9 ± 0.2 | 0.9 ± 0.1 | 0.5 ± 0.1 | 0.5 ± 0.0 |
824(pIPA3) | 43.0 ± 1.3 | 0.1 ± 0.0 | 5.1 ± 0.1 | 8.0 ± 0.1 | 0.8 ± 0.1 | 1.0 ± 0.1 | 0.5 ± 0.0 |
All results shown are means ± standard deviations from triplicate experiments. ND, not determined.