Table 4.
Enzyme activities in the recombinant strains
| Enzyme | Sp acta (mU/mg protein) |
|
|---|---|---|
| Acidogenic | Solventogenic | |
| CoAT in: | ||
| WT 824 | 1.7 ± 1.2 | 105 ± 19.0 |
| 824(pIPA3) | 40.1 ± 4.2 | 458 ± 48.7 |
| PJC4BK(pIPA3-Cm2) | 25 ± 5.8 | 109 ± 21.7 |
| AADC in: | ||
| WT 824 | 28.7 ± 15.3 | 105.6 ± 34.7 |
| 824(pIPA3) | 56.7 ± 20.8 | 243.3 ± 15.3 |
| PJC4BK(pIPA3-Cm2) | 39.6 ± 13.0 | 120.0 ± 20.0 |
| SADH (NADPH dependent)b in: | ||
| WT 824 | ND | ND |
| 824(pIPA3) | 1,910 ± 190 | 7,470 ± 690 |
| PJC4BK(pIPA3-Cm2) | 421 ± 51 | 1,320 ± 190 |
a
All results shown are means ± standard deviations from triplicate experiments. AADC activities are expressed in U/mg protein. ND, not detected.
b
NADH-dependent SADH activity was not detected in all strains.